Winston Kalengo will miss the Zambia-Cameroon clash.
The striker from AC Leopards in Congo-Brazzaville limped into camp with an injury.
Zambia team manager Stanley Kaseko said despite travelling with the team for the Zimbabwe friendly, it was decided to release Kalengo.
In addition, eight home-based players have been dropped after Zambia’s 1-0 friendly loss on Saturday away to Zimbabwe in Harare.
Midfielder Kelvin Mubanga, defender’s Lawrence Chungu and Billy Mutale including striker Patson Daka have failed to make the cut.
The Power Dynamos quartet were dropped together with Jack Chirwa of Green Buffaloes, Nkwazi striker Justin Shonga, Zanaco midfielder Richard Kasonde and Nkana defender Donashano Malama.
Ronald Kampamba, who received a late call-up, has made the cut.
Meanwhile, Zambia have arrived in Kampala ahead of Tuesday’s final friendly match there against Uganda.
Zambia will fly out of Kampala on Wednesday for Cameroon ahead of their November 12 date in a 2018 World Cup Group B qualifier in Limbe.
Goalkeepers: Jacob Banda (Zesco United), Allan Chibwe (Power Dynamos)
Defenders: Simon Silwimba, Kondwani Mtonga (both Zesco United), Ziyo Tembo, Fackson Kapumbu, George Chilufya (all Zanaco), Adrian Chama (Green Buffaloes)
Midfielders: Misheck Chaila (Zesco United), Roderick Kabwe, Charles Zulu (both Zanaco), Fwayo Tembo (Power Dynamos)
Striker: Ronald Kampamba (Nkana)
Goalkeeper: Kennedy Mweene (Mamelodi Sundowns, South Africa)
Defenders: Kabaso Chongo (TP Mazembe, DR Congo), Stopilla Sunzu (Lille, France)
Midfielders: Chisamba Lungu (FC Ural, Russia), Nathan Sinkala, Rainford Kalaba (both TP Mazembe, DR Congo)
Strikers: Winston Kalengo (AC Leopards, Congo-Brazzaville), Collins Mbesuma (Highlands Park, South Africa), Evans Kangwa (Gaziantepspor, Turkey), Rodgers Kola (Hapoel Ra’anana, Israel)